Amity Lipidomics Research Facility (ALRF), was established at the Amity University, Haryana in 2018, with funding from Department of Science and Technology (DST-FIST) Gov. of India and Amity University, Haryana.

The purpose of establishing this facility is to provide the scientific community (within Amity University and external researchers, both academia and industry) with state-of-the-art mass spectrometric techniques for separation, identification and quantitation of biomolecules like lipids, metabolites and proteins.

The Missions of ALRF are

  • ALRF provides comprehensive, qualitative and quantitative profiling of lipid molecular species with high sample throughput using mass spectrometric technologies
  • The services include help with the experimental design, analysis and data interpretation
  • ALRF provides training to students/researchers/industry on general principles of lipidomics and proteomics through workshops organized at the regular intervals throughout the year
  • ALRF aspires to develop new analytical methods for different types of sample analysis to make full use of the technology for exploring new dimensions in research

Lipidomics

Amity Lipidomics Research Facility houses a triple stage quadrupole/linear ion trap mass spectrometer, Q-Trap 4500 (SCIEX), equipped with ion sources (turbo V) Electrospray ionization (ESI), and Atmospheric pressure chemical ionization (APCI), autosampler, column oven with HPLC (Exion LC-AC) system. This instrument provides necessary resolution and sensitivity for a broad range of qualitative and quantitative lipid and metabolite analyses required for exhaustive characterization of lipid signaling molecules and metabolic pathways. We utilize ESI technique for generation of singly charged molecular ions with excellent concentration sensitivity and different scanning modes like precursor ion, neutral loss or MS/MS scan for detection of all species within a lipid/metabolite class. Separation and scanning of sample extracts in various scan modes give a complete and comprehensive profile of all molecular species in different lipid classes. Identification and quantification of unknown and known lipids/metabolites in biological samples such as cell extracts, tissue preparations, plasma, urine and saliva samples by using Data dependent (DDA) and data independent (DIA) analysis are done.

ALRF focuses on targeted and non-targeted analysis of lipids/metabolites. Depending on the lipid class and scientific question, analytical methods with (LC-MS/MS) or without (shotgun lipidomics) are used. LipidView and MultiQuant software are used for data processing.

Technology used for Lipid Profiling

We use a triple quad Q-TRAP 4500 (SCIEX) mass spectrometer with electrospray ionization source (ESI). Q-Trap 4500 functions like a standard triple quad and also doubles as a linear ion trap (LIT), a proprietary technology that can perform a multitude of additional workflows and is coupled to a HPLC system, Exion LC-AC (SCIEX). Direct infusions as well as LC-MS/MS methods are used for targeted and untargeted analysis of lipids/metabolites. ESI ionizes the lipid molecules, and forms protonated molecular ions ([M+H]+) and adduct molecular ions ([M+NH4]+, [M+Na]+, and [M+K]+) in ESI positive mode (ESI+). Deprotonated molecular anions ([M-H]-) and adduct anions ([M+Cl]- and [M+acetate]-) are formed ESI negative scan mode (ESI-). Fragmentation is done by collision activation and collision-induced dissociation (CID) by applying collision induced gas like (N2/Ar/He).

For targeted analysis we use Multiple Reactions Monitoring (MRM) scan mode or advanced scheduled MRM scan mode.

We use Information Dependent Acquisition (IDA) method for untargeted lipid molecules, where Precursor ion (Prec) and Neutral loss (NL) scans are performed that are specific to particular lipid classes; some classes are analyzed with Precursor scans, and others are analyzed with Neutral loss scans. The updated IDA method is capable for the profiling the lipids/metabolites in a single injection using additional enhanced product ion scan mode, which generate MS/MS spectrum that is used for library search or LipidView search for further confirmation.

Proteomics

ALRF provides service for targeted Proteomics analysis with focus on molecular weight confirmation, identification and their quantitation in complex biological samples. For Proteomics data analysis online Mascot and ProteinPilot 5.0 software are used for confirmation and structure identification of proteins molecules.

Application Support

  • Sample preparation
  • Mass spectrometric analysis
  • Data processing

Amity Lipidomics Research Facility (ALRF)

User Charges List

Equipment Experiment Amity University Academic& Research Institutions Private Industries/other Laboratories
UHPLC** Mass spectrometry 2. 3. 4. 5. 6. 1. ESI-MS based analysis Nominal mass/MW. Determination* Rs.400
per sample
Rs.800
per sample
Rs.1500
per sample
2. MS/MS analysis* Rs. 300 (per Precursor ion) Rs.600
(per Precursor ion)
Rs.1500
(per Precursor ion)
3. UPLC-MS analysis (Qualitative) Rs. 1000
(per Precursor ion)
Rs.2000
(per Precursor ion)
Rs.4000
(per Precursor ion)
4. UPLC-MS/MS Analysis (Qualitative) Rs.2500/sample (5 peaks, Rs 200 per additional peak) Rs.5000/ sample (5 peaks, Rs 300 per additional peak) Rs.10000/ sample (5 peaks, Rs 400 per additional peak)
5. UPLC-MS/MS for Targeted analysis of Lipids/Small molecules Rs 1000/ MRM/analytical lipid unit Rs 2500/ MRM/analytical lipid unit Rs 4000/ MRM/analytical lipid unit
6. UPLC-MS/MS for Targeted analysis of Lipids/Small molecules including data analysis by MultiQuant Software Rs 1500/ MRM/analytical lipid unit Rs 4000/ MRM/analytical lipid unit Rs 5000/ MRM/analytical lipid unit
7. UPLC-MS/MS for Untargeted analysis of Lipids/Small molecules Rs.1500/sample Rs.2500/ sample Rs.5000/ sample
8. UPLC-MS/MS for untargeted analysis of Lipids/Small molecules including data analysis by Lipid view Software Rs.2500/ sample Rs.5,000/ sample Rs.10,000/ sample

*Experiment no.1. and 2. would be done only for pure compound.

The facility organizes short term courses/ workshops on general principles of lipidomics and proteomics at regular intervals throughout the year to students and researchers, both from academia and industry.

Short-term Course on ‘‘General Principles of Lipidomics and Proteomics’’ at Amity Lipidomics Research Facility (ALRF) Amity University Haryana and Sciex, Gurgaon, Haryana 25th -29th March 2019

Amity Lipidomics Research Facility (ALRF) Amity University Haryana and Sciex, Gurgaon, Haryana organized the Short-term Course on ‘‘General Principles of Lipidomics and Proteomics’’ from 25 to 29 March 2019 at Amity University Haryana. In this workshop, the basic principles applications of liquid chromatography-mass spectrometry in the field of lipidomics and proteomics were taught with emphasis on QTRAP and TOF technology. Nine candidates participated from different institutions including JNU, AUH, ICAR, NIPGR, and ICGEB, Delhi.

The course instructor was Dr Faraz Rashid (Application Support Scientist Sciex Centre of Excellence, Gurgaon, Haryana). Lipidomic analysis using QTRAP Technology was presented by Dr Ujjaini Dasgupta (Co-ordinator) Asst. Prof. AIB/AIISH, AUH. The event was organized and co-ordinated by Dr Ujjaini Dasgupta and Mr Shiv Nandan (Technical Officer) AIB/AIISH, AUH, supported by Prof. Rajendra Prasad (Director, AIB/AIISH, AUH).

Director, of Amity Institute of Biotechnology and Amity Institute of Integrative Sciences and Health, AUH

Prof. Rajendra Prasad

Director,
AIB/AIISH, AUH

Email: rprasad@ggn.amity.edu

Eminent Scientist, Prof. Rajendra Prasad, is the Director of Amity Institute of Biotechnology and Amity Institute of Integrative Sciences and Health, AUH. Dr Prasad is a former Professor & Dean of School of Life Sciences at Jawaharlal Nehru University (JNU), New Delhi.

He is a leading molecular mycologist and has done trend setting research in the area of antifungal clinical drug resistance.

Dr. Ujjaini Dasgupta

Co-ordinator, (Assistant Professor)
AIB/AIISH, AUH

Email: udasgupta@ggn.amity.edu

Dr Ujjaini Dasgupta is an Assistant professor at Amity Institute of Biotechnology and Amity Institute of Integrative Sciences and Health, AUH. She got her postdoctoral training at University of Massachusetts Medical School Worcester, USA, where she worked on the intricate role of sphingolipids in phototransduction and GPCR signaling.

Currently, Ujjaini and her group are trying to decode the complex relation and interplay of sphingolipids in cancer biology.

Mr. Shiv Nandan

Technical Staff, (Technical Officer)

Email: snandan@ggn.amity.edu

Currently working as a technical officer in the Amity Lipidomics Research Facility (ALRF), Amity Institute of Biotechnology/Amity Institute of Integrative Science and Health, AUH.

Shiv Nandan has previously worked as a Project Assistant in the Department of Sophisticated Analytical Instrument Facility (SAIF) at the CSIR Central Drug Research Institute Lucknow. His research activities focus on HPLC/UHPLC, LC-MS and MS/MS analysis for identification and quantification of secondary metabolites and lipids in plants and animals sample to characterize the bioactive entities. He has wide experience in implementation of mass-spectrometry based methods for secondary metabolites and lipid profiling.

How to submit sample in ALRF

For Submission of lipid samples at ALRF, please contact Dr. Ujjaini Dasgupta/Mr. Shiv Nandan, at lipidomicauh@gmail.com

Send the samples on

Mr. Shiv Nandan (Technical Officer)
Lab No.117A
Amity Lipidomics Research Facility
Amity Institute of Biotechnology/
Amity Institute of Integrative Science and Health,
Amity University, Haryana 122413

For Submission of lipid samples at ALRF, Please fill Sample Submission Form and send it to Dr. Ujjaini Dasgupta/Mr. Shiv Nandan, at lipidomicauh@gmail.com

Contact us

Dr. Ujjaini Dasgupta
Mr Shiv Nandan

Amity Lipidomics Research Facility (ALRF),
Amity Institute of Biotechnology Department/
Amity Institute of Integrative Science and Health,

Amity University, Manesar Gurugram, Haryana.

Email: lipidomicauh@gmail.com

Working Hours (Mon - Fri) 09:00AM to 05:30PM