Dr. Nandlal Choudhary

Assistant Professor-II

Amity Institute Of Virology & Immunology

Plant Molecular Biology
Amity Institute of Virology and Immunology (AIVI)

Amity University, Sector-125, Noida

PhD: (2006) Indian Agricultural Research Institute, New Delhi.
10 Years
Recombinant DNA Technology, Frontier in Virology, Systemic Virology, Comprehensive Molecular Biology, Applied Virology, Essentials of Biochemistry, Viral Diseases of crop plants, Basics of Virology, Viral Disease Management, Translational Proteomics, Plant Physiology, Phytopathology, Epidemiology.
Plant Molecular Virology, Virus-host interaction
Plant Molecular Virology, Plant Molecular Biology and Biochemistry
02
Life Member in
1. Member of the Indian Virological Society from 2016
2. Asian PGPR society of sustainable Agriculture from 2016
3. International society for infectious disease from 2016
1. Choudhary Nandlal, Avijit Roy, Leon M. G, G. Wei, M. K. Nakhla, L. Levy, R. H. Brlansky (2017) Production of mono- and polyclonal antibodies of Citrus leprosis virus C2 and its application in diagnostic assays using triple antibody sandwich ELISA and immunocapture RT-PCR. J. Virological Methods, 243: 177-181.
2. Choudhary Nandlal, G. Wei, A. Govindarajulu, Avijit Roy, M. K. Nakhla, L.Levy, R. H. Brlansky (2015) Development of specific primers and probe for detection of Citrus leprosis virus C in one-step real-time reverse-transcription polymerase chain reaction assays. J. Virological Methods, 224:105-109.
3. Choudhary Nandlal, Roy A, Govindrajulu A, Nakhla M. K, Levy L and Brlansky R. H (2014) Production of monoclonal antibodies for detection of Citrus leprosis virus C in enzyme-linked immuno- assays and immunocapture reverse transcription-polymerase chain reaction. J. Virological Methods, 206, 144-149.
4. Choudhary Nandlal, Roy A, Guillermo L.M, Picton D.D, Wei G, Nakhla M.K, Levy L, and Brlansky R.H (2013) Immunodiagnosis of Citrus leprosis virus C using a polyclonal antibody to an expressed putative coat protein. J. Virological Methods, 193(2):548-553.
5. Choudhary Nandlal, Kapoor H.C. and Lodha M.L (2008) Cloning and expression of antiviral/ribosome-inactivating protein from Bougainvillea xbuttiana. Journal of Biosciences, 33 (1), 91-101.
6. Choudhary Nandlal, Yadav O.P. and Lodha M.L. (2008) Ribonuclease, deoxyribonuclease and antiviral activity of Escherichia coli-expressed Bougainvillea xbuttiana antiviral protein 1. Biochemistry Moscow, 73 (3), 273-277.
7. Choudhary Nandlal, Sairam R.K and Tyagi A (2005) Expression of ?1-pyrroline-5-carboxylate synthetase gene during drought in rice (Oryzasativa L.).Indian J. Biochemistry & Biophysics, 42(6), 366-370.
8. Roy A, Choudhary Nandlal, Guillermo L.M, Shao J, Govindarajulu A, Achor D, Wei G, Picton D.D, Levy L, Nakhla M.K, Hartung J.S, Brlansky R.H (2013) A novel virus of the genus Cilevirus causing symptoms similar to citrus leprosis. Phytopathology, 103(5):488-500. (Cover page article)
9. Avijit Roy, Choudhary Nandlal, J.S. Hartung and R.H. Brlansky (2013) The prevalence of the Citrus tristeza virus trifoliate resistant breaking genotype among Puerto Rican isolates. Plant Disease, 97 (9), 1227-1234.
10. G. Ananthakrishnan, Choudhary Nandlal, Avijit Roy, E. Postnikova, J.S. Hartung, A. L. Stone, V.D. Damsteegt, W.L. Schneider and R.H. Brlansky (2013) Development of primers and probes for genus and species specific detection of ‘Candidatus Liberibacter species’ by real-time PCR. Plant Disease, 97 (9), 1235-1243.
11. Avijit Roy, Andrew L. Stone, Jonathan Shao, Gabriel Otero-Colina, G. Wei, Choudhary Nandlal, Diann Achor, L. Levy, M. K. Nakhla, John S. Hartung, William L. Schneider and R. H. Brlansky (2015) Identification and molecular characterization of nuclear Citrus leprosis virus, an unassigned Dichorhavirus genus associated with citrus leprosis disease in Mexico. Phytopathology, 105: 564-575.
12. Mangrauthia S.K, Choudhary Nandlal and Tyagi A (2008) Cloning and characterization of drought responsive partial gene sequence(s) from Oryza sativa L. subsp. Indica. Indian J. Biochemistry & Biophysics, 45 (6), 387-392.
13. M.L. Lodha, Choudhary Nandlal, G.K. Mahapatro, B. Singh and G.P. Gupta (2011) Purification and evaluation of antiviral proteins from Bougainvillea x buttiana against Helicoverpa armigera. Indian Journal of Agricultural Sciences, 81 (1): 74-78. (Highlighted as News in Life Science Weekly, March 15th, 2011).
14. Roy A, Stone A, Otero-Colina G, Wei G, Choudhary Nandlal, Achor D, Shao J, Levy L, Nakhla M.K, Hollingsworth C.R, Hartung J.S, Schneider W.L, Brlansky R.H (2013) Genome assembly of citrus leprosis virus nuclear type reveals a close association with orchid fleck virus. Genome Announcement, 1(4): e00519-13.
15. Guillermo León, Avijit Roy, Nandlal Choudhary, Ronald Brlansky (2014) Citrus leprosis virus cytoplasmic type 2 (CiLV-C2) detection in Meta and Casanare States. Corpoica Cienc. Tecnol. Agropecu, 15 (2) 207-217.
16. Guillermo L.M, Avijit Roy, Choudhary Nandlal, Brlansky R.H. (2017). Citrus leprosis transmission by Brevipalpus yothersi mites through non citrus hosts. Corpoica Cienc Tecnol Agropecuaria, Mosquera (Colombia), 18(2): 307-319.
17. Guillermo L.M, Avijit Roy, Choudhary Nandlal, Brlansky R.H. (2017). Transmission parameters of citrus leprosis virus by Brevipalpus yothersi (Acari: Tenuipalpidae). Revista Colombiana de Entomología 43 (2): 215-222.
18. Damini Diksha, Susheel Kumar Sharma, Baby Wangkhem, Sumitra Phurailatpam, Yanglem Herojit Singh, Nandlal Choudhary, Virendra Kumar Baranwal (2024) Simplified extraction protocol of citrus tissues for rapid detection of ‘Candidatus Liberibacter asiaticus’ using isothermal recombinase polymerase amplification assay and its application for prevalence studies. South African Journal of Botany, 165, 517-525.
19. Damini Diksha, V. Kavi Sidharthan, Pankhuri Singhal, Nandlal Choudhary, Susheel Kumar Sharma, Sajad Un Nabi & Virendra Kumar Baranwal (2023). Multigene sequence analysis reveals occurrence of genetic variability in Candidatus Liberibacter asiaticus associated with Huanglongbing of Western and North-Eastern India". Indian Phytopathology 76, 261–272.
20. Jaiswal, D., Sidharthan, V.K., Sharma, S.K., Richa Rai, Nandlal Choudhary, Ghosh, A & V.K. Baranwal (2021). Candidatus Liberibacter asiaticus manipulates the expression of vitellogenin, cytoskeleton, and endocytotic pathway-related genes to become circulative in its vector, Diaphorina citri (Hemiptera: Psyllidae). 3Biotech, 11, 88.
21. Chandrasen Pandey, Neeraj Baghel, Malay Kishore Dutta, Ashish Srivastava & Nandlal Choudhary (2021) Machine learning approaches for automatic diagnosis of chlorosis in vigna mungo. Multimedia tools and applications. 80, 13407–13427.
22. Rakesh Chandra Joshi, Manoj Kaushik, Malay Kishore Dutta, Ashish Srivastava & Nandlal Choudhary (2021). Automatic analysis and viral disease diagnosis using deep learning in Vigna Mungo plant. Ecological Informatics, 61: 101197.
23. Nandlal Choudhary, Madan Lal Lodha and Virendra K Baranwal (2020) The role of enzymatic activities of antiviral proteins from plants for action against plant pathogens. 3Biotech, 10: 505.
24. Choudhary Nandlal, Avijit Roy, Leon M. G, G. Wei, M. K. Nakhla, L. Levy, R. H. Brlansky (2017) Production of mono- and polyclonal antibodies of Citrus leprosis virus C2 and its application in diagnostic assays using triple antibody sandwich ELISA and immunocapture RT-PCR. J. Virological Methods, 243: 177-181.

Book Chapters
1. Megha Khanna, Pooja Kumari, Tejaswini Sharma, Nandlal Choudhary, Lovelin Kaushal, and L.P. Awasthi (2023) Viral disease of Maize (Corn). Book: Viral diseases of field and horticultural crops. Pages 87-92 Publisher Elsevier. ISSN 9780323908993
2. Tara-Kay L. Jones, Julio S. Bernal, Raul F. Medina, Megha Khanna, Pooja Kumari, Tejaswini Sharma, and Nandlal Choudhary (2023) Viral Diseases of Oats. Book: Viral diseases of field and horticultural crops. Pages 63-70 Publisher Elsevier. ISSN 9780323908993
3. Houda Kawas, Filiz Ertunç, Ali Karanfil, Filiz Randa Zelyüt, Nandlal Choudhary (2023) Characterization of viruses infecting rose. Page no.749-754. Book: Viral diseases of field and horticultural crops. Publisher Elsevier. ISSN 9780323908993
4. Aniket Angira, Tejaswini Sharma, Nandlal Choudhary, Narayan Rishi, H.N.Verma and L.P.Awasthi (2023) Viral diseases of field and horticultural crops. Page no. 15-21. Book: Viral diseases of field and horticultural crops. Publisher Elsevier. ISSN 9780323908993.
5. Nandlal Choudhary (2021) Management of plant viruses through host RNAi defense mechanism. Pp-206-221. Books “Innovative approaches in diagnosis and management of crop diseases” published by CRC Press, Taylor and Francis group, Florida, USA.. ISBN: 9781774630266.
6. Nandlal Choudhary and Shobith Suresh (2020) Plant translation factors and virus resistance. Pp 657-669. Books Applied Plant Virology: Advances, Detection, and Antiviral Strategies. Publisher Elsevier. ISBN: 978-0-12-818654-1.
7. Nandlal Choudhary, Pooja Kumari and Soumya Panda (2020) RNA plant viruses: Biochemistry, replication and molecular genetics. Pp 183-195. Books Applied Plant Virology: Advances, Detection, and Antiviral Strategies. Publisher Elsevier. ISBN: 978-0-12-818654-1.
8. Nandlal Choudhary and Narayan Rishi (2016) Transboundary diseases and pests and their impact on agriculture and agroforestry. Pp. 1-18. Book: Plant pathogens and their management. Aavishkar Publishers, Distributors, Jaipur Rajasthan, India. (ISBN 9788179105382)
9. Nandlal Choudhary, Aruna Jangid and Shikha Dhatwalia (2017) Direct and indirect methods of gene transfer in plant. Plant Biotechnology: Principles, Techniques, and Applications, By Apple Academic Press, New Jersey, USA. E-Book ISBN: 9781315213095. Pp 1-26.
Awards & Honors:
• JRF in Life Sciences, UGC, New Delhi
• JRF in Biochemistry, IARI, New Delhi
• NET in Life Sciences, CSIR, New Delhi
• NET in Biochemistry-Plant Sciences, ICAR, New Delhi
• Certificate for active participation, Krishi Vigyan Mela and Horticulture Shows, IARI, New Delhi
Amity Institute of Virology and Immunology (AIVI)
Amity University, Sector-125, Noida