Amity Institute of Biotechnology Organized workshop on Introduction to basic and advanced concepts of downstream techniques” as part of the GE Healthcare Foundation workshop on Basic Protein Purification and Characterization on 23rd and 24th November 2020 by Dr. Siva Sivapathasekaran and Dr. Vaibhav Singh Bais, Lead Trainer, Cytiva (formerly GE Healthcare Life Sciences).
The Main Objective of the training was to introduce students to the essential chromatographic methods used in protein purification and help students design strategies for protein purification.
The two sessions on 23rd November were conducted by Dr. Vaibhav Singh Bais, Lead trainer, Cytiva. He introduced students to the fundamentals of Ion Exchange Chromatography and Size exclusion chromatography emphasizing on the principle, technique, equipment setup and data analysis associated with it.
The sessions on 24th November were conducted by Dr. Siva, lead trainer, Cytiva. His first session included discussion of the principle and applications of affinity chromatography citing examples like protein A and Protein G affinity towards IgG, IMAC for His-tagged protein purification etc. His second session was focussed on designing the purification strategies for protein of interests. He discussed the three-stage purification strategy, namely capture, intermediate purification and polishing, and linking of chromatographic techniques with each step.
Total 23 students and 2 faculties attended the session.